Disease Mechanisms and Targeted Therapies

Disease Mechanisms and Targeted Therapies

RECOMBINANT PROTEINS AND MONOCLONAL ANTIBODIES

The class of therapeutics called ”Biologics” include recombinant proteins, monoclonal antibodies, mRNAs, live cells, vaccines and recombinant viruses. Major biologics are monoclonal antibodies and recombinant proteins that are approved for the treatment of a variety of diseases, ranging from those that treat rare patients wirh rare but devastating genetic disorders to those treating hundreds of thousands of patients for some cancers and multiple sclerosis or even millions of patients for diseases such as asthma and rheumatoid arthritis.

Our laboratory is primarily interested in the exploration of home-designed recombinant proteins, monoclonal antibodies, and mRNAs for treatment of major diseases such as cancer and atherosclerosis. We select promising cell surface targets and design targeting tools such as ligands, soluble receptors and antibodies for targeted therapies.

Öztürk Grup

Öztürk Grup

We developed technologies such as monoclonal antibody production by hybridomas, recombinant protein production by synthetic genes in mammalian cells such as Hek293 and CHO, as well as protein purification by chromatography and biochemical characterization of proteins.

Antibodies and recombinant proteins offer a wide area of application in different domains of life sciences including medicine, pharmacy, veterinary medicine, microbiology, botaanic and agriculture. We welcome collaborations from all life science investigators and industrial partners for their needs in recombinant proteins and monoclonal antibodies.

Currently, we are producing monoclonal antibodies for diagnostic use in clinical pathology for cancer markers such as p53, p40, beta-catenin, Napsin A, cytokeratin 5 and PDL-1.  We are also producing chimeric proteins for engaging macrophages to eliminate atherosclerosis plaques.

Öztürk Grup

Prof. Dr. Mehmet Öztürk Prof. Dr. Mehmet Öztürk Laboratory Director mehmet.ozturk@tinaztepe.edu.tr

He is currently a lecturer at Izmir Tınaztepe University Faculty of Medicine and the director of IZTU Galen Research Center.

He was born in 1952 in Bolu-Mudurnu. He graduated from Gazi University Faculty of Pharmacy in 1974. He worked as a Pharmacist Intern at Paris Hospitals (Assistance Publique-Hopitaux de Paris) in France between 1977-1985, and received his Clinical Biochemistry and Immunology Specialization and Biochemistry Doctorate degrees from the University of Paris. Between 1985 and 1992, he worked as a Post-Doctoral Researcher and Lecturer at Harvard Medical School and Massachusetts General Hospital in the USA. In 1992, he became the Founding Director of the Molecular Oncology Laboratory at the Center Leon Berard Cancer Center in Lyon, France, and worked as INSERM Research Director at the same center between 1992-1995. In 1995, he was invited to Bilkent University as the Head of the Department to establish the Department of Molecular Biology and Genetics; He continued this duty until 2007. During the same period (1997-2013), he worked as the Founding Director of Bilkent University BilGen Genetics and Biotechnology Center. Between 2007 and 2012, he worked simultaneously as INSERM Research Director at Institut Albert Bonniot in Grenoble, France. Between September 2013 and February 2018, he worked as the Founding Director of Izmir International Biomedicine and Genome Institute affiliated to Dokuz Eylul University. He transformed this institution into Izmir Biomedicine and Genome Center, which is Turkey's first national public research institution in the field of health, within the scope of Law No. 6550 in 2018, and managed this center until 2021.

Prof. Dr. Mehmet Öztürk is known for his translational research to understand the genetic and molecular characteristics of cancer, especially liver cancer. More than one hundred scientific articles have been published and over 10,000 references have been made to them. His H-Factor is 46. As a Pharmacist specializing in Molecular Biology, Prof. Dr. Mehmet Öztürk has worked for many years for the production of biotechnological drugs based on gene engineering in our country.

Prof. Öztürk is a member of TÜBA, Academy of Sciences, EMBO and TWAS academies and is the recipient of the TÜBİTAK-TWAS Science Award and the Koç Foundation Health Award (as the founder of Bilkent MBG). He is married and he has two daughters.

Asst. Prof Bahriye Karakaş Köse Asst. Prof Bahriye Karakaş Köse bahriye.karakas@tinaztepe.edu.tr

Place of birth: Kırcaali. She earned her bachelor's degree in Molecular Biology and Genetics from Istanbul University and completed her master's degree through the Erasmus Mundus program in Animal Breeding and Genetics at AgroParisTech and the Swedish University of Agricultural Sciences (SLU). She completed her PhD in the Department of Molecular Biology, Genetics, and Bioengineering at Sabancı University’s Faculty of Engineering and Natural Sciences, continued her postdoctoral research there, and later worked at Eutropics Pharmaceuticals Inc. focusing on hybridoma technology and antibody research.

She joined Prof. Dr. Mehmet Öztürk’s team in 2020 and continues her work on the development and production of monoclonal antibodies. She currently serves as a Assistant Professor in the Medical Laboratory Techniques program at Izmir Tınaztepe University.

 

Phd. Saidu Sani Phd. Saidu Sani

 

  Dr. Saidu SANI is a lecturer in the Department of Biochemistry at Alex-Ekwueme Federal Ndufu Alike University (Ebonyi, Nigeria). Born in Maiduguri, Nigeria, Dr. Sani has an impressive academic background. He earned a National Diploma in Science Laboratory Technology with Distinction from Federal Polytechnic Bida, followed by a B.Tech in Biochemistry with First Class Honors and an M.Tech in Biochemistry, both from Federal University of Technology, Minna.

He completed his Ph.D. in Cellular and Molecular Biology at the University of Strasbourg, France, where his research investigated the roles of α5β1 integrin and p53 signaling pathways in glioblastoma resistance to Temozolomide. In 2024, Dr. Sani received the 2216-B TÜBİTAK-TWAS Postdoctoral Research Fellowship and currently continues his postdoctoral research at the Galen Research Center on the project “Targeting EPH Receptors in Cancer Using Recombinant Fusion Proteins.”

Expert Erhan Bal Expert Erhan Bal erhan.bal@tinaztepe.edu.tr

His place of birth is Düzce. He received his undergraduate degree in Biology from Ege University and his master’s degree in Biotechnology from Izmir Institute of Technology. He left Sabancı University Faculty of Engineering and Natural Sciences while continuing his PhD in Molecular Biology, Genetics and Bioengineering. He joined Mehmet Öztürk's team in 2018. He carries out DNA and protein-based biotechnological R&D studies in the laboratory.

 Sıla
 Kahyaoğlu Sıla Kahyaoğlu Lecturer sila.kahyaoglu@tinaztepe.edu.tr

She was born in Ankara. After completing her undergraduate studies in Biology at Kırıkkale University, she obtained her master’s degree in the Department of Pathology at the Faculty of Veterinary Medicine. During this period, she gained experience in the Histopathology unit of a central research laboratory. She gained her first work experience as a Laboratory Supervisor in the wet laboratories of the Faculty of Engineering at İzmir University of Economics. In 2018, she joined the Öztürk Lab at the İzmir Biomedical and Genome Center as a researcher and continues as the laboratory supervisor in Prof. Dr. Mehmet Öztürk’s team. She is involved in histopathology studies. In 2024, she started working as a Lecturer in the Department of Pathology Techniques at İzmir Tınaztepe University, School of Health Services.

Asst. Prof. Şule Aydın Saydam Asst. Prof. Şule Aydın Saydam Postdoctoral Researcher sule.aydin@tinaztepe.edu.tr

 

  She completed her undergraduate education at Ege University, Faculty of Science, Department of Biochemistry with a Biotechnology option, and her master’s and doctoral studies at Eskişehir Osmangazi University, Faculty of Medicine, Department of Medical Pharmacology. During her undergraduate studies, she worked on controlled drug delivery systems, and in her master’s and doctoral research, she focused on behavioral models in experimental animals and medical treatments for central nervous system disorders.

In 2024, she joined Prof. Dr. Mehmet Öztürk’s team at the Galen Research Center, where she continues her molecular biology-based research on therapeutic strategies using recombinant protein technologies.

Asst. Prof. Umut Ekin Asst. Prof. Umut Ekin Postdoctoral Researcher umut.ekin@tinaztepe.edu.tr

 

   He completed his undergraduate and master’s education in the Department of Molecular Biology and Genetics at İzmir Institute of Technology. He carried out his doctoral studies under the supervision of Prof. Dr. Mehmet Öztürk at Dokuz Eylül University’s İzmir International Biomedicine and Genome Institute, focusing on identifying new drug targets for liver cancer in the Molecular Biology and Genetics program.

As a researcher at the Galen Research Center, he participates in projects aimed at biotechnological drug design and the expression of designed recombinant proteins in mammalian cells, as well as the assessment of their efficacy. In 2024, he started his position as an Assistant Professor in the Medical Laboratory Techniques Department at İzmir Tınaztepe University, School of Health Sciences.

 

Phd. Latife Merve Oktay Çelebi Phd. Latife Merve Oktay Çelebi Postdoctoral Researcher latife.celebi@tinaztepe.edu.tr

She completed her undergraduate education in Biology Teaching at Balıkesir University, Necatibey Faculty of Education, and her master’s degree in the Molecular Biology program at Celal Bayar University, Department of Biology. She carried out her doctoral studies at Ege University, Faculty of Medicine, Department of Medical Biology, within the scope of the YÖK 100/2000 Molecular Pharmacology and Drug Research program, focusing on determining the cellular effects of plant-derived bioactive molecules and drug combinations on pancreatic cancer, as well as their impact on gene expression levels in cancer-related signaling pathways. In February 2023, she joined Prof. Dr. Mehmet Öztürk’s team and currently works as a postdoctoral researcher at the Galen Research Center, participating in studies to determine the bioactivity of developed monoclonal antibodies and recombinant proteins.

Öztürk Lab Alumni 

Postdoctoral Researchers 

  • PhD. Bahriye Karakaş Köse
  • PhD Aybike Erdoğan Atay
  • PhD Umut Ekin
  • PhD. Buse Kayhan
  • PhD Özden ÖZ

PhD Students

  • PhD Aybike Erdoğan ATAY

Master’s Students

  • Aslı Babayakalı, MSc

Undergraduate Students (Supported by 2247-C Intern Researcher Scholarship Program – STAR)

  • İrem Atabay
  • Hüseyin Bal
  • Esra Sultan Demir
  • Gözde Dilek Yalavuz 
  • Egemen Keleş

Undergraduate Students Who Completed Summer Internship

  • Tunç Ünal
  • Ayşenur Tezcan
  • Sude Naz Ünlü
  • Betül Balcı
  • İrem Tınaz
  • Melis Sert
  • Yağmur Oğuz
  • Doğa Sertoğlu

Öztürk Lab Alumni 

Postdoctoral Researchers 

  • PhD. Bahriye Karakaş Köse
  • PhD Umut Ekin
  • PhD. Buse Kayhan
  • PhD Özden ÖZ

PhD Students

  • PhD Aybike Erdoğan ATAY

Undergraduate Students (Supported by 2247-C Intern Researcher Scholarship Program – STAR)

  • İrem Atabay
  • Hüseyin Bal
  • Esra Sultan Demir
  • Gözde Dilek Yalavuz 
  • Egemen Keleş

Undergraduate Students Who Completed Summer Internship

  • Tunç Ünal
  • Ayşenur Tezcan
  • Sude Naz Ünlü
  • Betül Balcı
  • İrem Tınaz
  • Melis Sert
  • Yağmur Oğuz
  • Doğa Sertoğlu
  1. Batur T, Argundogan A, Keles U, Alotaibi H, Senturk S and Ozturk M. AXL knock-out in SNU475 hepatocellular carcinoma cells provides evidence for lethal effect associated with G2 arrest and polyploidization. Int J Mol Sci 2021. doi: 10.3390/ijms222413247.
  2. Oz O, Iscan E, Batur. T, Ozturk M. 3D Organoid modelling of hepatoblast-like and mesenchymal-like hepatocellular carcinoma cell lines. Hepatoma Res 2021: 7:60.10.20517/2394-5079.2021.43.
  3. Gungor MZ, Uysal M, Ozturk M, Senturk S. Systematic Analysis of Cytostatic TGF-Beta Response in Mesenchymal-Like Hepatocellular Carcinoma Cell Lines. J Gastrointest Cancer 2021 Aug 31. doi: 10.1007/s12029-021-00704-z. Epub ahead of print. PMID: 34463913.
  4. Ekin U, Yuzugullu H, Ozen C, Korhan P, Bagirsakci E, Yilmaz F, Yuzugullu OG, Uzuner H, Alotaibi H, Kirmizibayrak PB, Atabey N, Karakülah G, Ozturk M. Evaluation of ATAD2 as a Potential Target in Hepatocellular Carcinoma. J Gastrointest Cancer 2021 Nov 5. doi: 10.1007/s12029-021-00732-9. Epub ahead of print. PMID: 34738187.
  5. Iscan E, Ekin U, Yildiz G, Oz O, Keles U, Suner A, Cakan-Akdoga G, Ozhan G, Nekulova M, Vojtesek B, Uzuner H, Karakülah G, Alotaibi H, Ozturk M. TAp73β Can Promote Hepatocellular Carcinoma Dedifferentiation. Cancers (Basel) 2021 Feb 13;13(4):783.
  6. Kalyoncu, M., Demirci, D., Eris, S., Dayanc, B., Cakiroglu, E., Basol, M., Uysal, M., Cakan-Akdogan, G., Liu, F., Ozturk, M., Karakülah, G. and Senturk, S. (2025), Escape from TGF-β-induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells. Mol Oncol, 19: 2594-2618. https://doi.org/10.1002/1878-0261.70021
  7. Balcı Özyurt, A., Erkekoğlu, P., Zeybek, N. D., Aşcı, A., Yaman, Ü., Oflaz, O., Kızılgun, M., Iscan, E., Batur, T., Ozturk, M., Koçer-Gümüşel, B. (2023). Toxic effects of Aroclor 1254 on rat liver and modifying roles of selenium. International Journal of Environmental Health Research, 33(12), 1289–1304. https://doi.org/10.1080/09603123.2023.2223470
  8. Kalyoncu, S., Yilmaz, S., Kuyucu, A.Z. Ozturk, M. et al. Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastoris. Sci Rep 13, 5224 (2023).
  9. Akdeniz, G. Y., AKGÜN, H., BİNGÖL ÖZAKPINAR, Ö., Duracik, M., Ozturk, M., Iscan, E., ... Basoglu, F.(2022). Synthesis and Studies of Anticancer and Antimicrobial Activity of New Phenylurenyl Chalcone Derivatives. LETTERS IN DRUG DESIGN & DISCOVERY , vol.19, no.6, 500-519.
  10. Hiz S, Kiliç S, Bademci G, Karakulak T, Erdoğan A, Özden B, Eresen Ç, Erdal E, Yiş U, Tekin M, Karakülah G, Karaca E, Öztürk M. VARS1 mutations associated with neurodevelopmental disorder are located on a short amino acid stretch of the anticodon-binding domain. Turk J Biol. 2022 Dec 5;46(6):458-464. doi: 10.55730/1300-0152.2631. PMID: 37529793; PMCID: PMC10388123.
  11. Kılıç, S., Esmen, K., Oto, A. M., Kose, T. B., Sever-Bahçekapılı, M., Eren-Koçak, E., Demir, Ş., Hız, A. S., Karakülah, G., Bağrıyanık, H. A., Öztürk, M., & Diril, M. K. (2024). Characterization of a novel neurodevelopmental rare disease caused by a mutation within the autophagy gene ATG9B, BioRxiv, 2024.10.28.620020. https://doi.org/10.1101/2024.10.28.620020

ONGOING PROJECTS

1)Project Title: Evaluation of the ENBT1 carrier protein as a potential target in bladder cancer

Project Type: TÜBİTAK 1001

Project Leader: Prof. Dr. Mehmet ÖZTÜRK

 

2) Project Title: Targeting Eph Receptors in Cancer with Recombinant Fusion Proteins

Project Type: TÜBİTAK 1001

Project Leader: Prof. Dr. Mehmet ÖZTÜRK

 

3)Project Title: Target-Specific Pan-Cancer Therapies (PANTER)

Project Type: TÜBİTAK 1004

Implementing Institution: İzmir Biotechnology and Genomics Centre (İBG)

Contributing Institution: İzmir Tınaztepe University

 

COMPLETED PROJECTS

1)Project Name: Development and Production of Monoclonal Antibodies for Lung Carcinoma Frequently Used in Medical Pathology

Project Type: TÜBİTAK 1003

Project Leader: Prof. Dr. Mehmet ÖZTÜRK

 

2) Project Title: Phagocytosis-Triggering Chimeric Proteins Against Atherosclerosis

Project Type: TÜBİTAK 1001

Project Leader: Prof. Dr. Mehmet ÖZTÜRK

Postdoctoral researchers and postgraduate student candidates must apply to infogalen@tinaztepe.edu.tr with their CV and letter of intent.

Izmir Tinaztepe University

Galen Research Center
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